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Product Information

  • Product Name ELK Biotechnology Zebrafish GnRH(Gonadotropin Releasing Hormone) ELISA Kit
  • Catalog No. ELK9172
  • Pack Size 96T/48T
  • Brand ELK Biotechnology Co. Ltd.
  • Product Detail This ELISA kit applies to the in vitro quantitative determination of Zebrafish Gonadotropin Releasing Hormone concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Features

    Sensitivity: 0.1 ng/mL

    Standard: 16 ng/mL

    Detection Range: 0.25-16 ng/mL

    Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;

Alternate Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Zebrafish GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.

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