Product Information

Test principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Immunoglobulin G2 (IgG2) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Immunoglobulin G2 (IgG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Please contact our technical support expert at support@clementiabiotech.com for the latest date sheet & the kit manual.

The product is for laboratory use only (RUO).