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The product is for lab research use only.
Test Principle: This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to alpha-hydroxybutyric Acid. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for alpha-hydroxybutyric Acid is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain alpha-hydroxybutyric Acid and HRP conjugated alpha-hydroxybutyric Acid antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of alpha-hydroxybutyric Acid. You can calculate the concentration of alpha-hydroxybutyric Acid in the samples by comparing the OD of the samples to the standard curve.
