Please contact our technical support expert at support@clementiabiotech.com for the latest product description.
The product is for lab research use only.
Test Principle: This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IFI16/p16. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IFI16/p16 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IFI16/p16 and HRP conjugated IFI16/p16 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IFI16/p16. You can calculate the concentration of IFI16/p16 in the samples by comparing the OD of the samples to the standard curve.
