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Test Principle: The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to H-1, make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for H-1 is added to each Microplate well and incubated, so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain H-1 and HRP conjugated H-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of H-1 is determined by comparing with the CUTOFF value.