Detection: 15.63 - 1000pg/mL
Sample: Serum, Plasma, Cell supernatant.
Target Name: 11-DH-TXB2
This ELISA Kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat 11-DH-TXB2. During the reaction, Rat 11-DH-TXB2 in the sample or standard competes with a fixed amount of Rat 11-DH-TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat 11-DH-TXB2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Rat 11-DH-TXB2 in the samples is then determined by comparing the OD of the samples to the standard curve.