Sensitivity: 0.94ng/mL
Detection: 1.56 - 100ng/mL
Sample: Serum, Plasma, Cell supernatant.
Target Name: 20S-PSM
UNIProt ID:
This ELISA Kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat 20S-PSM. During the reaction, Rat 20S-PSM in the sample or standard competes with a fixed amount of Rat 20S-PSM on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat 20S-PSM. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Rat 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.
