Elabscience TUNEL assay kit is designed to specifically detect DNA breaks produced during apotosis process in cells. The kits are techncial davantage of highly sensitivity. With advnatge of low background staining but bright positive staining, the kit can detect apoptosis at single cell level. The kit's smart reagents can label apoptotic cells leaving necrotic cells. The detetcion is fast with only 1-2 hours require to complete the expermient. The kit can be applied to detection of apoptosis in frozen or paraffin sections, as well as in cultured adherent or suspended cells. The kit is available in different versions to meet the researcher's need.
TUNEL Assay Kits
| Catalogue No. | Assay Kit and its Discription | |
| E-CK-A320 |
One-step TUNEL In Situ Apoptosis Kit (Green, FITC) Detection principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. General Information: Application: DNA Fragmentation The kit is for lab research use only |
|
| E-CK-A321 |
One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) Detection principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. General Information: Application: DNA Fragmentation The kit is for lab research use only |
|
|
E-CK-A322 |
One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594) Detection principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. General Information: Application: DNA Fragmentation The kit is for lab research use only |
|
| E-CK-A324 |
One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647) Detection principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. General Information: Application: DNA Fragmentation The kit is for lab research use only |
|
| E-CK-A325 | One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555)
Detection principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. General Information: Application: DNA Fragmentation The kit is for lab research use only |
Feel free to ask Query
