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F-TESTO Free TestosteroneELK Biotechnology Human F-Testo(Free Testosterone) ELISA Kit
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Product Information

  • Product Name ELK Biotechnology Human F-Testo(Free Testosterone) ELISA Kit
  • Catalog No. ELK9028
  • Pack Size 96T/48T
  • Brand ELK Biotechnology Co. Ltd.
  • Product Detail This ELISA kit applies to the in vitro quantitative determination of Human Free Testosterone concentrations in serum, plasma, other biological fluids
  • Features

    Sensitivity: 0.48 pg/mL

    Standard: 100 pg/mL

    Detection Range: 1.57-100 pg/mL

    Assay Length: 2h

Assay Type: Competitive Inhibition

Research Area: Endocrinology;Reproductive science;Hormone metabolism;

Alternate Names: F-TESTO; Free Testosterone

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F-Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human F-Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

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