Assay Type: Sandwich
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
Alternate Names: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.
